An important cartographical rendering of the region of Asia Minor is shown in the Map of the Ottoman State printed in Athens in 1861. A series of studies, bound together thanks to the interest and care of the founder of the Library, Joannes Gennadius, offer early testimonies about the Hellenism of Asia Minor. Studies concerning the language of Pontus and Cappadocia are supplemented with books on the history of Phocaea, Erythrai (Litri), and Pergamon, as well as on the geography of Pontus, Chaldea, and Sinope.

Multiculturalism is evident through a plethora of religious and educational karamanli and francochiot publications, most of which belonged to collector and bibliographer Eugène Dalleggio and were donated to the Gennadius Library in 1990. An extremely rare item is a Turkish-language religious publication in Armenian characters printed in Smyrna in 1842.

A unique historical document is the Black Book of Persecutions and Testimonies of Hellenism in Turkey, 1914 – 1918 published by the Ecumenical Patriarchate.